From the 30th of June, Bendigo Health's COVID-19 testing drive-through on McLaren street will close.
Residents will still be able to do walk-ins at the facility.
At it's peak, the site was swabbing 600 to 800 patients each day.
Bendigo Health regional vaccination and screening program manager Daniel Sheard said staff at the centre have done "wonderful job" working in difficult conditions.
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